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Anti-Human SPAG5 Polyclonal Antibody (HP190014)

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Vista general
Número de catálogoHP190014
Descripción
Anti-Human SPAG5 Polyclonal Antibody (HP190014) is a rabbit polyclonal antibody detecting SPAG5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human SPAG5 (Met976-Asp1098).
Objetivo Sperm-associated antigen 5, SPAG5, Astrin, Deepest, MAP126, Mitotic spindle-associated protein p126
Purificación Purified by antigen affinity column.
Número de acceso Q96R06
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Sperm-associated antigen 5 (SPAG5) is a ~134 kDa protein. Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes.

1. Mack, GJ. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 14434-9. PMID: 11724960
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3. Chu, X. et al. (2016) The Journal of biological chemistry 291, 20055-67. PMID: 27462074
4. Thein, KH. et al. (2007) The Journal of cell biology 178, 345-54. PMID: 17664331
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6. Dunsch, AK. et al. (2011) The Journal of cell biology 192, 959-68. PMID: 21402792
7. Kodani, A. et al. (2015) eLife 4. PMID: 26297806
8. Thedieck, K. et al. (2013) Cell 154, 859-74. PMID: 23953116
9. Cheng, TS. et al. (2008) The Journal of biological chemistry 283, 2454-64. PMID: 18055457
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human SPAG5 Polyclonal Antibody

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