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Anti-Human SPAG5 Polyclonal Antibody (HP190014)

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Aperçu
Numéro de catalogueHP190014
Description
Anti-Human SPAG5 Polyclonal Antibody (HP190014) is a rabbit polyclonal antibody detecting SPAG5 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human SPAG5 (Met976-Asp1098).
Cible Sperm-associated antigen 5, SPAG5, Astrin, Deepest, MAP126, Mitotic spindle-associated protein p126
Purification Purified by antigen affinity column.
Numéro d'accès Q96R06
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Sperm-associated antigen 5 (SPAG5) is a ~134 kDa protein. Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes.

1. Mack, GJ. et al. (2001) Proceedings of the National Academy of Sciences of the United States of America 98, 14434-9. PMID: 11724960
2. Gruber, J. et al. (2002) Journal of cell science 115, 4053-9. PMID: 12356910
3. Chu, X. et al. (2016) The Journal of biological chemistry 291, 20055-67. PMID: 27462074
4. Thein, KH. et al. (2007) The Journal of cell biology 178, 345-54. PMID: 17664331
5. Du, J. et al. (2008) Biochemical and biophysical research communications 370, 213-9. PMID: 18361916
6. Dunsch, AK. et al. (2011) The Journal of cell biology 192, 959-68. PMID: 21402792
7. Kodani, A. et al. (2015) eLife 4. PMID: 26297806
8. Thedieck, K. et al. (2013) Cell 154, 859-74. PMID: 23953116
9. Cheng, TS. et al. (2008) The Journal of biological chemistry 283, 2454-64. PMID: 18055457
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human SPAG5 Polyclonal Antibody

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