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Anti-Human SPOUT1 Polyclonal Antibody (HD297014)

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Vista general
Número de catálogoHD297014
Descripción
Anti-Human SPOUT1 Polyclonal Antibody (HD297014) is a rabbit polyclonal antibody detecting SPOUT1. Suitable for Mouse and Human.
Highlights
  • ●Multi-Species — Cross-reactive for translational research.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human SPOUT1 (Thr74-Thr376).
Objetivo 28S rRNA (uridine-N(3))-methyltransferase, C9orf114, CENP-32, Centromere protein 32, EC:2.1.1.-, Kinetochore-associated protein, Methyltransferase C9orf114, Ribosomal RNA methyltransferase SPOUT1, SPOUT domain-containing methyltransferase 1, SPOUT1
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso Q5T280
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

28S rRNA (uridine-N(3))-methyltransferase (SPOUT1) is a ~42 kDa protein. S-adenosyl-L-methionine-dependent RNA methyltransferase. Specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase, ensuring proper spindle organization. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis.

1. Chen, J. et al. (2024) Science advances 10, eadr1743. PMID: 39671501
2. Dharmadhikari, AV. et al. (2025) Nature communications 16, 1703. PMID: 39962046
3. Ohta, S. et al. (2010) Cell 142, 810-21. PMID: 20813266
4. Ohta, S. et al. (2015) Molecular biology of the cell 26, 1225-37. PMID: 25657325
5. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233
Nota For research use only
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Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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