Anti-Human SPOUT1 Polyclonal Antibody (HD297014)

Name: Anti-Human SPOUT1 Polyclonal Antibody
Brand: abinScience
SKU: HD297014
Price: 168 USD
Availability: InStock

Fiche technique Partager le produit Copier les informations

Prix(USD) : --

Spécification :

50ug
100ug

Nombre :

Contactez-nous

Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HD297014

Description

Anti-Human SPOUT1 Polyclonal Antibody (HD297014) is a rabbit polyclonal antibody detecting SPOUT1. Suitable for Mouse and Human.

Highlights

●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human SPOUT1 (Thr74-Thr376).

Cible

28S rRNA (uridine-N(3))-methyltransferase, C9orf114, CENP-32, Centromere protein 32, EC:2.1.1.-, Kinetochore-associated protein, Methyltransferase C9orf114, Ribosomal RNA methyltransferase SPOUT1, SPOUT domain-containing methyltransferase 1, SPOUT1

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

Q5T280

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Contexte

28S rRNA (uridine-N(3))-methyltransferase (SPOUT1) is a ~42 kDa protein. S-adenosyl-L-methionine-dependent RNA methyltransferase. Specifically methylates the N3 position of a uridine in 28S rRNA. Required for association of the centrosomes with the poles of the bipolar mitotic spindle during metaphase, ensuring proper spindle organization. Also involved in chromosome alignment. May promote centrosome maturation probably by recruiting A-kinase anchor protein AKAP9 to centrosomes in early mitosis.

1. Chen, J. et al. (2024) Science advances 10, eadr1743. PMID: 39671501

2. Dharmadhikari, AV. et al. (2025) Nature communications 16, 1703. PMID: 39962046

3. Ohta, S. et al. (2010) Cell 142, 810-21. PMID: 20813266

4. Ohta, S. et al. (2015) Molecular biology of the cell 26, 1225-37. PMID: 25657325

5. Treiber, T. et al. (2017) Molecular cell 66, 270-284.e13. PMID: 28431233

Note

For research use only

Images

Reférences

Fiche technique

Anti-Human SPOUT1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Human SPOUT1 Polyclonal Antibody (HD297014)

Aperçu

Images

Reférences

Fiche technique

SDS