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Anti-Human SRSF10 Polyclonal Antibody (HF607014)

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Aperçu
Numéro de catalogueHF607014
Description
Anti-Human SRSF10 Polyclonal Antibody (HF607014) is a rabbit polyclonal antibody detecting SRSF10 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human SRSF10 (Met1-Ser121).
Cible Serine/arginine-rich splicing factor 10, 40 kDa SR-repressor protein, SRrp40, FUS-interacting serine-arginine-rich protein 1, Splicing factor SRp38, Splicing factor, arginine/serine-rich 13A, TLS-associated protein with Ser-Arg repeats, TASR, TLS-associated protein with SR repeats, TLS-associated serine-arginine protein, TLS-associated SR protein, SRSF10, FUSIP1, FUSIP2, SFRS13A, TASR
Purification Purified by antigen affinity column.
Numéro d'accès O75494
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Serine/arginine-rich splicing factor 10 (SRSF10) is a ~31 kDa protein. Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. Seems to interfere with the U1 snRNP 5'-splice recognition of SNRNP70. Required for splicing repression in M-phase cells and after heat shock. Also acts as a splicing factor that specifically promotes exon skipping during alternative splicing. Interaction with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing.

1. Cowper, AE. et al. (2001) The Journal of biological chemistry 276, 48908-14. PMID: 11684676
2. Shin, C. et al. (2002) Cell 111, 407-17. PMID: 12419250
3. Shin, C. et al. (2004) Nature 427, 553-8. PMID: 14765198
4. Xiao, W. et al. (2016) Molecular cell 61, 507-519. PMID: 26876937
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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C₂ (Working Conc.)
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Anti-Human SRSF10 Polyclonal Antibody

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