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Anti-Human SRY Polyclonal Antibody (HC271014)

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Aperçu
Numéro de catalogueHC271014
Description
Anti-Human SRY Polyclonal Antibody (HC271014) is a rabbit polyclonal antibody detecting SRY in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human SRY (Met1-Leu204).
Cible TDF, Sex-determining region Y protein, Testis-determining factor, SRY
Purification Purified by antigen affinity column.
Numéro d'accès Q05066
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

Sex-determining region Y protein (SRY) is a ~23 kDa protein. Transcriptional regulator that controls a genetic switch in male development. It is necessary and sufficient for initiating male sex determination by directing the development of supporting cell precursors (pre-Sertoli cells) as Sertoli rather than granulosa cells. Involved in different aspects of gene regulation including promoter activation or repression. Binds to the DNA consensus sequence 5'-[AT]AACAA[AT]-3'. SRY HMG box recognizes DNA by partial intercalation in the minor groove and promotes DNA bending.

1. Murphy, EC. et al. (2001) Journal of molecular biology 312, 481-99. PMID: 11563911
2. Oh, HJ. et al. (2006) Molecular and cellular endocrinology 247, 47-52. PMID: 16414182
3. Polanco, JC. et al. (2007) Developmental biology 302, 13-24. PMID: 16996051
4. Desclozeaux, M. et al. (1998) The Journal of biological chemistry 273, 7988-95. PMID: 9525897
5. Ferrari, S. et al. (1992) The EMBO journal 11, 4497-506. PMID: 1425584
6. Phillips, NB. et al. (2004) Biochemistry 43, 7066-81. PMID: 15170344
7. Giese, K. et al. (1994) Proceedings of the National Academy of Sciences of the United States of America 91, 3368-72. PMID: 8159753
8. King, CY. et al. (1993) Proceedings of the National Academy of Sciences of the United States of America 90, 11990-4. PMID: 8265659
Note For research use only.
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Reférences
Fiche technique
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-Human SRY Polyclonal Antibody

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