Anti-Human SSBP1 Polyclonal Antibody (HC422014)

Name: Anti-Human SSBP1 Polyclonal Antibody
Brand: abinScience
SKU: HC422014
Price: 96 USD
Availability: InStock

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Vista general

Número de catálogo

HC422014

Descripción

Anti-Human SSBP1 Polyclonal Antibody (HC422014) is a rabbit polyclonal antibody detecting SSBP1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human SSBP1 (His16-Glu148).

Objetivo

Single-stranded DNA-binding protein, mitochondrial, Mt-SSB, MtSSB, PWP1-interacting protein 17, SSBP1, SSBP

Purificación

Purified by antigen affinity column.

Número de acceso

Q04837

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Single-stranded DNA-binding protein, mitochondrial (SSBP1) is a ~17 kDa protein. Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.

1. Oliveira, MT. et al. (2011) The Journal of biological chemistry 286, 40649-58. PMID: 21953457

2. van Loon, B. et al. (2013) DNA repair 12, 177-87. PMID: 23290262

3. Del Dotto, V. et al. (2020) The Journal of clinical investigation 130, 108-125. PMID: 31550240

4. Korhonen, JA. et al. (2003) The Journal of biological chemistry 278, 48627-32. PMID: 12975372

5. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897

6. Ciesielski, GL. et al. (2015) The Journal of biological chemistry 290, 28697-707. PMID: 26446790

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-SSBP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: A549 cell lysate
Lane 2: SW620 cell lysate

Referencias

Datasheet

Anti-Human SSBP1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

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