Anti-Human SSBP1 Polyclonal Antibody (HC422014)

Name: Anti-Human SSBP1 Polyclonal Antibody
Brand: abinScience
SKU: HC422014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HC422014

Description

Anti-Human SSBP1 Polyclonal Antibody (HC422014) is a rabbit polyclonal antibody detecting SSBP1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human SSBP1 (His16-Glu148).

Cible

Single-stranded DNA-binding protein, mitochondrial, Mt-SSB, MtSSB, PWP1-interacting protein 17, SSBP1, SSBP

Purification

Purified by antigen affinity column.

Numéro d'accès

Q04837

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Single-stranded DNA-binding protein, mitochondrial (SSBP1) is a ~17 kDa protein. Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.

1. Oliveira, MT. et al. (2011) The Journal of biological chemistry 286, 40649-58. PMID: 21953457

2. van Loon, B. et al. (2013) DNA repair 12, 177-87. PMID: 23290262

3. Del Dotto, V. et al. (2020) The Journal of clinical investigation 130, 108-125. PMID: 31550240

4. Korhonen, JA. et al. (2003) The Journal of biological chemistry 278, 48627-32. PMID: 12975372

5. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897

6. Ciesielski, GL. et al. (2015) The Journal of biological chemistry 290, 28697-707. PMID: 26446790

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-SSBP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: A549 cell lysate
Lane 2: SW620 cell lysate

Reférences

Fiche technique

Anti-Human SSBP1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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