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| カタログ番号 | HC422014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human SSBP1 Polyclonal Antibody (HC422014) is a rabbit polyclonal antibody detecting SSBP1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human SSBP1 (His16-Glu148). | ||||||||
| ターゲット | Single-stranded DNA-binding protein, mitochondrial, Mt-SSB, MtSSB, PWP1-interacting protein 17, SSBP1, SSBP | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q04837 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Single-stranded DNA-binding protein, mitochondrial (SSBP1) is a ~17 kDa protein. Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair. 1. Oliveira, MT. et al. (2011) The Journal of biological chemistry 286, 40649-58. PMID: 21953457 2. van Loon, B. et al. (2013) DNA repair 12, 177-87. PMID: 23290262 3. Del Dotto, V. et al. (2020) The Journal of clinical investigation 130, 108-125. PMID: 31550240 4. Korhonen, JA. et al. (2003) The Journal of biological chemistry 278, 48627-32. PMID: 12975372 5. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897 6. Ciesielski, GL. et al. (2015) The Journal of biological chemistry 290, 28697-707. PMID: 26446790 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-SSBP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: A549 cell lysate
Lane 2: SW620 cell lysate
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