Anti-Human SSBP1 Polyclonal Antibody (HC422014)

Name: Anti-Human SSBP1 Polyclonal Antibody
Brand: abinScience
SKU: HC422014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HC422014

Описание

Anti-Human SSBP1 Polyclonal Antibody (HC422014) is a rabbit polyclonal antibody detecting SSBP1 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pongo abelii.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human SSBP1 (His16-Glu148).

Цель

Single-stranded DNA-binding protein, mitochondrial, Mt-SSB, MtSSB, PWP1-interacting protein 17, SSBP1, SSBP

Очистка

Purified by antigen affinity column.

Номер доступа

Q04837

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Single-stranded DNA-binding protein, mitochondrial (SSBP1) is a ~17 kDa protein. Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays a crucial role during mtDNA replication by stimulating the activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.

1. Oliveira, MT. et al. (2011) The Journal of biological chemistry 286, 40649-58. PMID: 21953457

2. van Loon, B. et al. (2013) DNA repair 12, 177-87. PMID: 23290262

3. Del Dotto, V. et al. (2020) The Journal of clinical investigation 130, 108-125. PMID: 31550240

4. Korhonen, JA. et al. (2003) The Journal of biological chemistry 278, 48627-32. PMID: 12975372

5. Korhonen, JA. et al. (2004) The EMBO journal 23, 2423-9. PMID: 15167897

6. Ciesielski, GL. et al. (2015) The Journal of biological chemistry 290, 28697-707. PMID: 26446790

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-SSBP1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: A549 cell lysate
Lane 2: SW620 cell lysate

Ссылки

Даташит

Anti-Human SSBP1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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