Anti-Human STUB1 Polyclonal Antibody (HP128014)

Name: Anti-Human STUB1 Polyclonal Antibody
Brand: abinScience
SKU: HP128014
Price: 96 USD
Availability: InStock

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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HP128014

Description

Anti-Human STUB1 Polyclonal Antibody (HP128014) is a rabbit polyclonal antibody detecting STUB1 in ELISA, IHC, WB. Suitable for Chicken, Human, Mouse, and Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human STUB1 (Ser23-Tyr303).

Cible

Antigen NY-CO-7, CHIP, CLL-associated antigen KW-8, STUB1, Carboxy terminus of Hsp70-interacting protein, STIP1 homology and U box-containing protein 1, E3 ubiquitin-protein ligase CHIP, RING-type E3 ubiquitin transferase CHIP

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9UNE7

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

E3 ubiquitin-protein ligase CHIP (STUB1) is a ~34 kDa protein. E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Plays a role in the maintenance of mitochondrial morphology and promotes mitophagic removal of dysfunctional mitochondria; thereby acts as a protector against apoptosis in response to cellular stress. Negatively regulates vascular smooth muscle contraction, via degradation of the transcriptional activator MYOCD and subsequent loss of transcription of genes involved in vascular smooth muscle contraction. Promotes survival and proliferation of cardiac smooth muscle cells via ubiquitination and degradation of FOXO1, resulting in subsequent repression of FOXO1-mediated transcription of pro-apoptotic genes. Ubiquitinates ICER-type isoforms of CREM and targets them for proteasomal degradation, thereby acts as a positive effector of MAPK/ERK-mediated inhibition of apoptosis in cardiomyocytes.

1. Ballinger, CA. et al. (1999) Molecular and cellular biology 19, 4535-45. PMID: 10330192

2. Connell, P. et al. (2001) Nature cell biology 3, 93-6. PMID: 11146632

3. Jiang, J. et al. (2001) The Journal of biological chemistry 276, 42938-44. PMID: 11557750

4. Matsumura, Y. et al. (2013) The Journal of biological chemistry 288, 31069-79. PMID: 23990462

5. Teng, Y. et al. (2015) Biochemical pharmacology 97, 518-530. PMID: 26265139

6. Li, F. et al. (2009) The Journal of biological chemistry 284, 20090-8. PMID: 19483080

7. Woo, CH. et al. (2010) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, 4917-28. PMID: 20724525

8. Chao, CN. et al. (2019) Journal of cellular physiology 234, 20128-20138. PMID: 30980393

10. Parsons, JL. et al. (2009) The EMBO journal 28, 3207-15. PMID: 19713937

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-STUB1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: L929 cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: A549 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Rat brain lysate

Reférences

Fiche technique

Anti-Human STUB1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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