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| カタログ番号 | HP128014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human STUB1 Polyclonal Antibody (HP128014) is a rabbit polyclonal antibody detecting STUB1 in ELISA, IHC, WB. Suitable for Chicken, Human, Mouse, and Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human STUB1 (Ser23-Tyr303). | ||||||||
| ターゲット | Antigen NY-CO-7, CHIP, CLL-associated antigen KW-8, STUB1, Carboxy terminus of Hsp70-interacting protein, STIP1 homology and U box-containing protein 1, E3 ubiquitin-protein ligase CHIP, RING-type E3 ubiquitin transferase CHIP | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9UNE7 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | E3 ubiquitin-protein ligase CHIP (STUB1) is a ~34 kDa protein. E3 ubiquitin-protein ligase which targets misfolded chaperone substrates towards proteasomal degradation. Plays a role in the maintenance of mitochondrial morphology and promotes mitophagic removal of dysfunctional mitochondria; thereby acts as a protector against apoptosis in response to cellular stress. Negatively regulates vascular smooth muscle contraction, via degradation of the transcriptional activator MYOCD and subsequent loss of transcription of genes involved in vascular smooth muscle contraction. Promotes survival and proliferation of cardiac smooth muscle cells via ubiquitination and degradation of FOXO1, resulting in subsequent repression of FOXO1-mediated transcription of pro-apoptotic genes. Ubiquitinates ICER-type isoforms of CREM and targets them for proteasomal degradation, thereby acts as a positive effector of MAPK/ERK-mediated inhibition of apoptosis in cardiomyocytes. 1. Ballinger, CA. et al. (1999) Molecular and cellular biology 19, 4535-45. PMID: 10330192 2. Connell, P. et al. (2001) Nature cell biology 3, 93-6. PMID: 11146632 3. Jiang, J. et al. (2001) The Journal of biological chemistry 276, 42938-44. PMID: 11557750 4. Matsumura, Y. et al. (2013) The Journal of biological chemistry 288, 31069-79. PMID: 23990462 5. Teng, Y. et al. (2015) Biochemical pharmacology 97, 518-530. PMID: 26265139 6. Li, F. et al. (2009) The Journal of biological chemistry 284, 20090-8. PMID: 19483080 7. Woo, CH. et al. (2010) FASEB journal : official publication of the Federation of American Societies for Experimental Biology 24, 4917-28. PMID: 20724525 8. Chao, CN. et al. (2019) Journal of cellular physiology 234, 20128-20138. PMID: 30980393 10. Parsons, JL. et al. (2009) The EMBO journal 28, 3207-15. PMID: 19713937 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-STUB1 polyclonal antibody at 1μg/ml on various samples.
Lane 1: L929 cell lysate
Lane 2: Hela cell lysate
Lane 3: NIH3T3 cell lysate
Lane 4: A549 cell lysate
Lane 5: PC-12 cell lysate
Lane 6: Mouse brain lysate
Lane 7: Rat brain lysate
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