









| Каталожный номер | HY373107 |
|---|---|
| Реактивность видов | Human |
| Применения | FCM |
| Хозяинский вид | Mouse |
| Клоональность | Monoclonal |
| Изотип | IgG2a, kappa |
| ID клона | SAA0113 |
| Проверенные приложения | FCM |
| Цель | Pancreatic carcinoma marker protein GA733-1, Tumor-associated calcium signal transducer 2, M1S1, TROP2, TACSTD2, Cell surface glycoprotein Trop-2, GA733-1, Membrane component chromosome 1 surface marker 1 |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >95% as determined by SDS-PAGE. |
| Очистка | Protein A/G purified from cell culture supernatant. |
| Номер доступа | P09758 |
| Форма | Liquid |
| Буфер хранения | 0.01M PBS pH 7.4 Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Фон | Tumor-associated calcium signal transducer 2 (TACSTD2/TROP2) is a ~35 kDa protein. May function as a growth factor receptor. TACSTD2 is the therapeutic target of sacituzumab govitecan (Trodelvy). |
| Примечание | For research use only. |

Detects Human TACSTD2/TROP2 in indirect ELISAs.

Flow-cytometry using anti-human TROP2 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TROP2 antibody monoclonal antibody (Catalog # HY373107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human TACSTD2 antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TACSTD2 antibody monoclonal antibody (Catalog # HY373107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human TACSTD2 antibody.PC-3 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TACSTD2 antibody monoclonal antibody (Catalog # HY373107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.

Flow-cytometry using anti-human TACSTD2 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human TACSTD2 monoclonal antibody (Catalog: HY373107) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.




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