Anti-Human TAF1 Polyclonal Antibody (HB919014)

Name: Anti-Human TAF1 Polyclonal Antibody
Brand: abinScience
SKU: HB919014
Price: 96 USD
Availability: InStock

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Обзор

Каталожный номер

HB919014

Описание

Anti-Human TAF1 Polyclonal Antibody (HB919014) is a rabbit polyclonal antibody detecting TAF1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human TAF1 (Ser635-Gly944).

Цель

p250, TAF(II)250, CCGS, BA2R, TAF1, Transcription initiation factor TFIID subunit 1, Cell cycle gene 1 protein, TAFII-250, TAF2A, CCG1, TBP-associated factor 250 kDa, Transcription initiation factor TFIID 250 kDa subunit, TAFII250

Очистка

Purified by antigen affinity column.

Номер доступа

P21675

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Transcription initiation factor TFIID subunit 1 (TAF1) is a ~214 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. TAF1 is the largest component and core scaffold of the TFIID complex, involved in nucleating complex assembly. TAF1 forms a promoter DNA binding subcomplex of TFIID, together with TAF7 and TAF2.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473

2. Wang, H. et al. (2014) Cell research 24, 1433-44. PMID: 25412659

3. Louder, RK. et al. (2016) Nature 531, 604-9. PMID: 27007846

4. Dikstein, R. et al. (1996) Cell 84, 781-90. PMID: 8625415

Примечание

For research use only.

Изображения

Western blot

Western blot analysis was performed using anti-TAF1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: 293T cell lysate
Lane 2: Jurkat cell lysate