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Anti-Human TBP Polyclonal Antibody (HB847014)

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Vista general
Número de catálogoHB847014
Descripción
Anti-Human TBP Polyclonal Antibody (HB847014) is a rabbit polyclonal antibody detecting TBP in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human TBP (Ala155-Phe335).
Objetivo TF2D, TATA-binding factor, GTF2D1, Transcription initiation factor TFIID TBP subunit, TFIID, TBP, TATA sequence-binding protein, TATA-box factor, TATA-box-binding protein
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P20226, P29037
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

TATA-box-binding protein (TBP) is a ~37 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TBP forms the TFIID-A module together with TAF3 and TAF5.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Kao, CC. et al. (1990) Science (New York, N.Y.) 248, 1646-50. PMID: 2194289
3. Peterson, MG. et al. (1990) Science (New York, N.Y.) 248, 1625-30. PMID: 2363050
4. Hoffman, A. et al. (1990) Nature 346, 387-90. PMID: 2374612
5. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682
6. Louder, RK. et al. (2016) Nature 531, 604-9. PMID: 27007846
Nota For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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