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Anti-Human TBP Polyclonal Antibody (HB847014)

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Aperçu
Numéro de catalogueHB847014
Description
Anti-Human TBP Polyclonal Antibody (HB847014) is a rabbit polyclonal antibody detecting TBP in ELISA, IHC, WB. Suitable for Human and Mouse.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactivité des espècesHuman, Mouse
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human TBP (Ala155-Phe335).
Cible TF2D, TATA-binding factor, GTF2D1, Transcription initiation factor TFIID TBP subunit, TFIID, TBP, TATA sequence-binding protein, TATA-box factor, TATA-box-binding protein
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P20226, P29037
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stabilité et stockage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Contexte

TATA-box-binding protein (TBP) is a ~37 kDa protein. The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription. TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC). The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13. The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C. TBP forms the TFIID-A module together with TAF3 and TAF5.

1. Chen, X. et al. (2021) Science (New York, N.Y.) 372. PMID: 33795473
2. Kao, CC. et al. (1990) Science (New York, N.Y.) 248, 1646-50. PMID: 2194289
3. Peterson, MG. et al. (1990) Science (New York, N.Y.) 248, 1625-30. PMID: 2363050
4. Hoffman, A. et al. (1990) Nature 346, 387-90. PMID: 2374612
5. He, Y. et al. (2016) Nature 533, 359-65. PMID: 27193682
6. Louder, RK. et al. (2016) Nature 531, 604-9. PMID: 27007846
Note For research use only.
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Formula
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