Anti-Human TFE3 Polyclonal Antibody (HB043014)

Name: Anti-Human TFE3 Polyclonal Antibody
Brand: abinScience
SKU: HB043014
Price: 96 USD
Availability: InStock

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SDS

Aperçu

Numéro de catalogue

HB043014

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human TFE3 (Asn185-Ser281).

Cible

TFE3, bHLHe33, BHLHE33, Transcription factor E3, Class E basic helix-loop-helix protein 33

Purification

Purified by antigen affinity column.

Numéro d'accès

P19532

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Transcription factor E3 (TFE3) is a ~61 kDa protein. Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes.

1. Beckmann, H. et al. (1990) Genes & development 4, 167-79. PMID: 2338243

2. Martina, JA. et al. (2014) Science signaling 7, ra9. PMID: 24448649

3. Willett, R. et al. (2017) Nature communications 8, 1580. PMID: 29146937

4. Mathieu, J. et al. (2019) Nature communications 10, 632. PMID: 30733432

5. Lawrence, RE. et al. (2019) Science (New York, N.Y.) 366, 971-977. PMID: 31672913

6. Malik, N. et al. (2023) Science (New York, N.Y.) 380, eabj5559. PMID: 37079666

7. Nardone, C. et al. (2023) Molecular cell 83, 57-73.e9. PMID: 36608670

Note

For research use only.

Images

Reférences

Fiche technique

Anti-Human TFE3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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