Anti-Human TFE3 Polyclonal Antibody (HB043014)

Name: Anti-Human TFE3 Polyclonal Antibody
Brand: abinScience
SKU: HB043014
Price: 96 USD
Availability: InStock

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HB043014

Реактивность видов

Human, Mouse

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human TFE3 (Asn185-Ser281).

Цель

TFE3, bHLHe33, BHLHE33, Transcription factor E3, Class E basic helix-loop-helix protein 33

Очистка

Purified by antigen affinity column.

Номер доступа

P19532

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Transcription factor E3 (TFE3) is a ~61 kDa protein. Transcription factor that acts as a master regulator of lysosomal biogenesis and immune response. Specifically recognizes and binds E-box sequences (5'-CANNTG-3'); efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFEB or MITF. Involved in the cellular response to amino acid availability by acting downstream of MTOR: in the presence of nutrients, TFE3 phosphorylation by MTOR promotes its inactivation. Upon starvation or lysosomal stress, inhibition of MTOR induces TFE3 dephosphorylation, resulting in transcription factor activity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression, thereby playing a central role in expression of lysosomal genes.

1. Beckmann, H. et al. (1990) Genes & development 4, 167-79. PMID: 2338243

2. Martina, JA. et al. (2014) Science signaling 7, ra9. PMID: 24448649

3. Willett, R. et al. (2017) Nature communications 8, 1580. PMID: 29146937

4. Mathieu, J. et al. (2019) Nature communications 10, 632. PMID: 30733432

5. Lawrence, RE. et al. (2019) Science (New York, N.Y.) 366, 971-977. PMID: 31672913

6. Malik, N. et al. (2023) Science (New York, N.Y.) 380, eabj5559. PMID: 37079666

7. Nardone, C. et al. (2023) Molecular cell 83, 57-73.e9. PMID: 36608670

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human TFE3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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