中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HA072014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human TIGAR Polyclonal Antibody (HA072014) is a rabbit polyclonal antibody detecting TIGAR in ELISA, IHC, WB. Suitable for Human.
Highlights
|
||||||||
| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human TIGAR (Met1-Arg270). | ||||||||
| ターゲット | Fructose-2, 6-bisphosphatase TIGAR, TP53-induced glycolysis and apoptosis regulator, TIGAR, C12orf5, TP53-induced glycolysis regulatory phosphatase | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q9NQ88 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
||||||||
| 製品使用情報 |
| ||||||||
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Fructose-2,6-bisphosphatase TIGAR is a ~30 kDa protein. Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate. Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production. Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death. Plays a role in promoting protection against cell death during hypoxia by decreasing mitochondria ROS levels in a HK2-dependent manner through a mechanism that is independent of its fructose-bisphosphatase activity. In response to cardiac damage stress, mediates p53-induced inhibition of myocyte mitophagy through ROS levels reduction and the subsequent inactivation of BNIP3. 1. Li, H. et al. (2009) The Journal of biological chemistry 284, 1748-54. PMID: 19015259 2. Bensaad, K. et al. (2006) Cell 126, 107-20. PMID: 16839880 3. Wanka, C. et al. (2012) The Journal of biological chemistry 287, 33436-46. PMID: 22887998 4. Bensaad, K. et al. (2009) The EMBO journal 28, 3015-26. PMID: 19713938 5. Cheung, EC. et al. (2013) Developmental cell 25, 463-77. PMID: 23726973 6. Ye, L. et al. (2013) Biochemical and biophysical research communications 437, 300-6. PMID: 23817040 7. Cheung, EC. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 20491-6. PMID: 23185017 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-TIGAR polyclonal antibody at 1ug/mL on various samples.
Lane 1: Raji cell lysate
Lane 2: HepG2 cell lysate
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
