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Каталожный номерHP219014
Описание
Anti-Human TMEM165 Polyclonal Antibody (HP219014) is a rabbit polyclonal antibody detecting TMEM165 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Реактивность видовHuman
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human TMEM165 (Gly34-Thr89).
Цель TPARL, TMEM165, Transmembrane protein PT27, Transmembrane protein TPARL, Transmembrane protein 165
Очистка Purified by antigen affinity column.
Номер доступа Q9HC07
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Putative divalent cation/proton antiporter TMEM165 is a ~34 kDa protein. Putative divalent cation:proton antiporter that exchanges calcium or manganese ions for protons across the Golgi membrane. Mediates the reversible transport of calcium or manganese to the Golgi lumen driven by the proton gradient and possibly the membrane potential generated by V-ATPase. Provides calcium or manganese cofactors to resident Golgi enzymes and contributes to the maintenance of an acidic luminal Golgi pH required for proper functioning of the secretory pathway. Promotes Ca(2+) storage within the Golgi lumen of the mammary epithelial cells to be then secreted into milk. The transport mechanism and stoichiometry remains to be elucidated.

1. Foulquier, F. et al. (2012) American journal of human genetics 91, 15-26. PMID: 22683087
2. Demaegd, D. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 6859-64. PMID: 23569283
3. Potelle, S. et al. (2016) Human molecular genetics 25, 1489-500. PMID: 27008884
4. Stribny, J. et al. (2020) The Journal of biological chemistry 295, 3865-3874. PMID: 32047108
Примечание For research use only.
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Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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