Anti-Human TP53BP1 IP Recombinant Antibody (SAA0236) (HC342013)

Name: Anti-Human TP53BP1 IP Recombinant Antibody (SAA0236)
Brand: abinScience
SKU: HC342013
Price: 126 USD
Availability: InStock

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Precio(USD):

Especificación:

20ug
50ug
100ug
1mg

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HC342013

Reactividad de especies

Human

Aplicaciones

ELISA, IF, IP

Especie huésped

Mouse

Clonalidad

Monoclonal

Isotipo

IgG2a

ID de clon

SAA0236

Objetivo

TP53-binding protein 1, TP53BP1, p53-binding protein 1, p53BP1, 53BP1

Nivel de endotoxinas

Please contact with the lab for this information.

Pureza

>95% as determined by SDS-PAGE.

Purificación

Protein A/G purified from cell culture supernatant.

Número de acceso

Q12888

RRID

Anti-Human TP53BP1 IP Antibody (SAA0236) (abinScience Cat# HC342013, RRID:AB_3729770)

Forma

Liquid

Buffer de almacenamiento

0.01M PBS pH 7.4

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:2000-1:20000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Fondo

TP53-binding protein 1 (TP53BP1) is a ~213 kDa protein. Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs.

1. Wang, B. et al. (2002) Science (New York, N.Y.) 298, 1435-8. PMID: 12364621

2. Botuyan, MV. et al. (2006) Cell 127, 1361-73. PMID: 17190600

3. Kang, Y. et al. (2011) Biochemical and biophysical research communications 404, 476-81. PMID: 21144835

4. Chapman, JR. et al. (2012) Journal of cell science 125, 3529-34. PMID: 22553214

5. Escribano-Díaz, C. et al. (2013) Molecular cell 49, 872-83. PMID: 23333306

6. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538

7. Drané, P. et al. (2017) Nature 543, 211-216. PMID: 28241136

8. Bigot, N. et al. (2019) eLife 8. PMID: 31135337

9. Swift, ML. et al. (2023) Nature structural & molecular biology 30, 1456-1467. PMID: 37696958

Nota

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Imágenes

SDS-PAGE

SDS-PAGE for Anti-Human TP53BP1 IP Antibody (SAA0236)

Referencias

Datasheet

Anti-Human TP53BP1 IP Recombinant Antibody (SAA0236).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo