Anti-Human TP53BP1 IP Recombinant Antibody (SAA0236) (HC342013)

Name: Anti-Human TP53BP1 IP Recombinant Antibody (SAA0236)
Brand: abinScience
SKU: HC342013
Price: 126 USD
Availability: InStock

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1mg

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HC342013

Reactivité des espèces

Human

Applications

ELISA, IF, IP

Espèce hôte

Mouse

Clonalité

Monoclonal

Isotype

IgG2a

ID de clone

SAA0236

Cible

TP53-binding protein 1, TP53BP1, p53-binding protein 1, p53BP1, 53BP1

Niveau d'endotoxines

Please contact with the lab for this information.

Pureté

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Numéro d'accès

Q12888

RRID

Anti-Human TP53BP1 IP Antibody (SAA0236) (abinScience Cat# HC342013, RRID:AB_3729770)

Forme

Liquid

Tampon de stockage

0.01M PBS pH 7.4

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:2000-1:20000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Contexte

TP53-binding protein 1 (TP53BP1) is a ~213 kDa protein. Double-strand break (DSB) repair protein involved in response to DNA damage, telomere dynamics and class-switch recombination (CSR) during antibody genesis. Plays a key role in the repair of double-strand DNA breaks (DSBs) in response to DNA damage by promoting non-homologous end joining (NHEJ)-mediated repair of DSBs and specifically counteracting the function of the homologous recombination (HR) repair protein BRCA1. In response to DSBs, phosphorylation by ATM promotes interaction with RIF1 and dissociation from NUDT16L1/TIRR, leading to recruitment to DSBs sites. Recruited to DSBs sites by recognizing and binding histone H2A monoubiquitinated at 'Lys-15' (H2AK15Ub) and histone H4 dimethylated at 'Lys-20' (H4K20me2), two histone marks that are present at DSBs sites. Required for immunoglobulin class-switch recombination (CSR) during antibody genesis, a process that involves the generation of DNA DSBs.

1. Wang, B. et al. (2002) Science (New York, N.Y.) 298, 1435-8. PMID: 12364621

2. Botuyan, MV. et al. (2006) Cell 127, 1361-73. PMID: 17190600

3. Kang, Y. et al. (2011) Biochemical and biophysical research communications 404, 476-81. PMID: 21144835

4. Chapman, JR. et al. (2012) Journal of cell science 125, 3529-34. PMID: 22553214

5. Escribano-Díaz, C. et al. (2013) Molecular cell 49, 872-83. PMID: 23333306

6. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538

7. Drané, P. et al. (2017) Nature 543, 211-216. PMID: 28241136

8. Bigot, N. et al. (2019) eLife 8. PMID: 31135337

9. Swift, ML. et al. (2023) Nature structural & molecular biology 30, 1456-1467. PMID: 37696958

Note

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Images

SDS-PAGE

SDS-PAGE for Anti-Human TP53BP1 IP Antibody (SAA0236)

Reférences

Fiche technique

Anti-Human TP53BP1 IP Recombinant Antibody (SAA0236).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

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