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Anti-Human 5T4 Recombinant Antibody (SAA0148), APC (HC281137)

Anti-Human 5T4 Recombinant Antibody (SAA0148), APC
Anti-Human 5T4 Recombinant Antibody (SAA0148), APC
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Especificación:
  • 50T
  • 100T
Número:
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Vista general
Número de catálogoHC281137
Reactividad de especiesHuman
AplicacionesFCM, IF, WB
Especie huéspedMouse
ClonalidadMonoclonal
IsotipoIgG1, kappa
ID de clonSAA0148
ConjugaciónAPC
Aplicaciones probadas FCM
Objetivo 5T4, TPBG, WAIF1, M6P1, 5T4 oncofetal trophoblast glycoprotein, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1
Nivel de endotoxinas Please contact with the lab for this information.
Productos asociados Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Número de acceso Q13641
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Estabilidad y almacenamiento Store at 4°C for 12 months. Protect from light. Do not freeze.
Fondo

Trophoblast glycoprotein (TPBG/5T4) is a ~46 kDa protein. May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Nota For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Imágenes
  • Anti-Human 5T4 Recombinant Antibody (SAA0148), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human ANGPT2 antibody. HUVEC cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human ANGPT2 monoclonal antibody (Catalog: HC281137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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