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Anti-Human 5T4 Recombinant Antibody (SAA0148), APC (HC281137)

Anti-Human 5T4 Recombinant Antibody (SAA0148), APC
Anti-Human 5T4 Recombinant Antibody (SAA0148), APC
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Aperçu
Numéro de catalogueHC281137
Reactivité des espècesHuman
ApplicationsFCM, IF, WB
Espèce hôteMouse
ClonalitéMonoclonal
IsotypeIgG1, kappa
ID de cloneSAA0148
ConjugaisonAPC
Applications testées FCM
Cible 5T4, TPBG, WAIF1, M6P1, 5T4 oncofetal trophoblast glycoprotein, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1
Niveau d'endotoxines Please contact with the lab for this information.
Produits associés Mouse IgG1, kappa Recombinant Isotype Control Antibody (MOPC-21), APC
Numéro d'accès Q13641
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 0.2% BSA, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Stabilité et stockage Store at 4°C for 12 months. Protect from light. Do not freeze.
Contexte

Trophoblast glycoprotein (TPBG/5T4) is a ~46 kDa protein. May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.

Note For flow cytometric staining, the suggested use of this reagent is 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application. For research use only.
Images
  • Anti-Human 5T4 Recombinant Antibody (SAA0148), APC

    Flow CytoMetry

    Flow-cytometry using APC anti-human ANGPT2 antibody. HUVEC cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an APC anti-human ANGPT2 monoclonal antibody (Catalog: HC281137, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
Recommandation

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