Anti-Human 5T4 Recombinant Antibody (SAA0571) (HC281207)

Name: Anti-Human 5T4 Recombinant Antibody (SAA0571)
Brand: abinScience
SKU: HC281207
Price: 191 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HC281207
Reactivité des espèces	Human
Applications	ELISA, FCM, SPR
Espèce hôte	Mouse
Clonalité	Monoclonal
Isotype	IgG2a
ID de clone	SAA0571
Applications testées	FCM
Cible	5T4, TPBG, WAIF1, M6P1, 5T4 oncofetal trophoblast glycoprotein, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Numéro d'accès	Q13641
Forme	Liquid
Tampon de stockage	0.01M PBS, pH 7.4, 0.09% Sodium azide. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Contexte	Trophoblast glycoprotein (TPBG/5T4) is a ~46 kDa protein. May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Note	For research use only.

Images

Bioactivity

Detects TPBG/5T4 in indirect ELISAs.
Bioactivity

Detects Recombinant Human TPBG/5T4 Protein, C-His (Cat No.: HC281011) in indirect ELISA.
Flow Cytometry

Flow-cytometry using anti-human TPBG antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TPBG antibody monoclonal antibody (Catalog # HC281207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human TPBG antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TPBG antibody monoclonal antibody (Catalog # HC281207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human TPBG antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human TPBG monoclonal antibody (Catalog: HC281207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.