Anti-Human 5T4 Recombinant Antibody (SAA0571) (HC281207)

Name: Anti-Human 5T4 Recombinant Antibody (SAA0571)
Brand: abinScience
SKU: HC281207
Price: 191 USD
Availability: InStock

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Каталожный номер	HC281207
Реактивность видов	Human
Применения	ELISA, FCM, SPR
Хозяинский вид	Mouse
Клоональность	Monoclonal
Изотип	IgG2a
ID клона	SAA0571
Проверенные приложения	FCM
Цель	5T4, TPBG, WAIF1, M6P1, 5T4 oncofetal trophoblast glycoprotein, Trophoblast glycoprotein, 5T4 oncofetal antigen, 5T4 oncotrophoblast glycoprotein, Wnt-activated inhibitory factor 1
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>95% as determined by SDS-PAGE.
Очистка	Protein A/G purified from cell culture supernatant.
Номер доступа	Q13641
Форма	Liquid
Буфер хранения	0.01M PBS, pH 7.4, 0.09% Sodium azide. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Фон	Trophoblast glycoprotein (TPBG/5T4) is a ~46 kDa protein. May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Примечание	For research use only.

Изображения

Bioactivity

Detects TPBG/5T4 in indirect ELISAs.
Bioactivity

Detects Recombinant Human TPBG/5T4 Protein, C-His (Cat No.: HC281011) in indirect ELISA.
Flow Cytometry

Flow-cytometry using anti-human TPBG antibody.HT-29 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TPBG antibody monoclonal antibody (Catalog # HC281207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human TPBG antibody.MCF7 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TPBG antibody monoclonal antibody (Catalog # HC281207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow CytoMetry

Flow-cytometry using anti-human TPBG antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human TPBG monoclonal antibody (Catalog: HC281207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.