Anti-Human TRIM13 Polyclonal Antibody (HF807014)

Name: Anti-Human TRIM13 Polyclonal Antibody
Brand: abinScience
SKU: HF807014
Price: 96 USD
Availability: InStock

Datasheet Compartir producto Copiar información

Precio(USD):

Especificación:

20ug
50ug
100ug

Número:

Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HF807014

Descripción

Anti-Human TRIM13 Polyclonal Antibody (HF807014) is a rabbit polyclonal antibody detecting TRIM13 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human TRIM13 (Met1-Arg153).

Objetivo

E3 ubiquitin-protein ligase TRIM13, 2.3.2.27, B-cell chronic lymphocytic leukemia tumor suppressor Leu5, Leukemia-associated protein 5, Putative tumor suppressor RFP2, RING finger protein 77, RING-type E3 ubiquitin transferase TRIM13, Ret finger protein 2, Tripartite motif-containing protein 13, TRIM13, LEU5, RFP2, RNF77

Purificación

Purified by antigen affinity column.

Número de acceso

O60858

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

E3 ubiquitin-protein ligase TRIM13 is a ~46 kDa protein. Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor. Also plays a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway.

1. Tomar, D. et al. (2012) Biochimica et biophysica acta 1823, 316-26. PMID: 22178386

2. Huang, B. et al. (2017) Molecular pharmacology 91, 307-316. PMID: 28087809

3. Hatchi, EM. et al. (2014) The international journal of biochemistry & cell biology 54, 217-22. PMID: 25088585

4. Tomar, D. et al. (2014) Cellular signalling 26, 2606-13. PMID: 25152375

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-TRIM13 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate

Referencias

Datasheet

Anti-Human TRIM13 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo