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Anti-Human TRMT5 Polyclonal Antibody (HD270014)

Anti-Human TRMT5 Polyclonal Antibody
Anti-Human TRMT5 Polyclonal Antibody
Prix(USD) :
Spécification :
  • 50ug
  • 100ug
Nombre :
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Aperçu
Numéro de catalogueHD270014
Description
Anti-Human TRMT5 Polyclonal Antibody (HD270014) is a rabbit polyclonal antibody detecting TRMT5. Suitable for Human.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human TRMT5 (Thr65-Lys475).
Cible EC:2.1.1.228, KIAA1393, M1G-methyltransferase, TRM5, TRMT5, tRNA (guanine(37)-N(1))-methyltransferase, tRNA [GM37] methyltransferase, tRNA methyltransferase 5 homolog
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès Q32P41
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

tRNA (guanine(37)-N(1))-methyltransferase (TRMT5) is a ~58 kDa protein. Involved in mitochondrial tRNA methylation. Specifically methylates the N1 position of guanosine-37 in various tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.

1. Powell, CA. et al. (2015) American journal of human genetics 97, 319-28. PMID: 26189817
Note For research use only
Images
  • Anti-Human TRMT5 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TRMT5 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: NIH3T3 cell lysate

Reférences
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
Recommandation

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