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| カタログ番号 | HB852013 | ||||||
|---|---|---|---|---|---|---|---|
| 種反応性 | Human | ||||||
| アプリケーション | ELISA, FCM, IHC, WB | ||||||
| 宿主種 | Mouse | ||||||
| クローン性 | Monoclonal | ||||||
| アイソタイプ | IgG2a, lambda | ||||||
| クローンID | SAA0401 | ||||||
| テスト済みアプリケーション | WB, FCM | ||||||
| ターゲット | Thyroid-stimulating hormone receptor, TSH-R, TSHR, Thyrotropin receptor, LGR3 | ||||||
| エンドトキシンレベル | Please contact with the lab for this information. | ||||||
| 純度 | >95% as determined by SDS-PAGE. | ||||||
| 精製 | Protein A/G purified from cell culture supernatant. | ||||||
| アクセッション番号 | P16473 | ||||||
| RRID | Anti-Human TSHR Antibody (SAA0401) (abinScience Cat# HB852013, RRID:AB_3725206) |
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| 形態 | Liquid | ||||||
| 保存バッファー | 0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. | ||||||
| 背景 | Thyrotropin receptor (TSHR/LGR3) is a ~86 kDa protein. Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. TSHR is coupled to G(s) proteins and mediates the activation of adenylate cyclase. This leads to the generation of cyclic adenosine monophosphate (cAMP), which in turn activates protein kinase A (PKA). PKA subsequently phosphorylates downstream targets involved in thyroid hormone biosynthesis and secretion, including thyroid peroxidase (TPO) and the sodium/iodide symporter (NIS). 1. Costagliola, S. et al. (2002) The EMBO journal 21, 504-13. PMID: 11847099 2. Nakabayashi, K. et al. (2002) The Journal of clinical investigation 109, 1445-52. PMID: 12045258 3. Faust, B. et al. (2022) Nature 609, 846-853. PMID: 35940205 4. Duan, J. et al. (2022) Nature 609, 854-859. PMID: 35940204 5. Morgan, SJ. et al. (2016) Thyroid : official journal of the American Thyroid Association 26, 1794-1803. PMID: 27638195 | ||||||
| 注意事項 | For research use only. | ||||||
| Isotype Control | Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023) |
Flow-cytometry using anti-human TSHR antibody.TSHR Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TSHR antibody monoclonal antibody (Catalog # HB852013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE for Anti-Human TSHR Antibody (SAA0401).
Western blot analysis was performed using anti-TSHR monoclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse ovary lysate
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