Anti-Human TSHR Recombinant Antibody (SAA0401) (HB852013)

Name: Anti-Human TSHR Recombinant Antibody (SAA0401)
Brand: abinScience
SKU: HB852013
Price: 126 USD
Availability: InStock

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Цена(USD):

Спецификация:

20ug
50ug
100ug
1mg

Количество:

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Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HB852013

Реактивность видов

Human

Применения

ELISA, FCM, IHC, WB

Хозяинский вид

Mouse

Клоональность

Monoclonal

Изотип

IgG2a, lambda

ID клона

SAA0401

Проверенные приложения

WB, FCM

Цель

Thyroid-stimulating hormone receptor, TSH-R, TSHR, Thyrotropin receptor, LGR3

Уровень эндотоксинов

Please contact with the lab for this information.

Чистота

>95% as determined by SDS-PAGE.

Очистка

Protein A/G purified from cell culture supernatant.

Номер доступа

P16473

RRID

Anti-Human TSHR Antibody (SAA0401) (abinScience Cat# HB852013, RRID:AB_3725206)

Форма

Liquid

Буфер хранения

0.01M PBS pH 7.4

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:2000-1:20000
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Фон

Thyrotropin receptor (TSHR/LGR3) is a ~86 kDa protein. Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. TSHR is coupled to G(s) proteins and mediates the activation of adenylate cyclase. This leads to the generation of cyclic adenosine monophosphate (cAMP), which in turn activates protein kinase A (PKA). PKA subsequently phosphorylates downstream targets involved in thyroid hormone biosynthesis and secretion, including thyroid peroxidase (TPO) and the sodium/iodide symporter (NIS).

1. Costagliola, S. et al. (2002) The EMBO journal 21, 504-13. PMID: 11847099

2. Nakabayashi, K. et al. (2002) The Journal of clinical investigation 109, 1445-52. PMID: 12045258

3. Faust, B. et al. (2022) Nature 609, 846-853. PMID: 35940205

4. Duan, J. et al. (2022) Nature 609, 854-859. PMID: 35940204

5. Morgan, SJ. et al. (2016) Thyroid : official journal of the American Thyroid Association 26, 1794-1803. PMID: 27638195

Примечание

For research use only.

Isotype Control

Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)

Изображения

Flow Cytometry

Flow-cytometry using anti-human TSHR antibody.TSHR Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TSHR antibody monoclonal antibody (Catalog # HB852013 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for Anti-Human TSHR Antibody (SAA0401).
Western blot

Western blot analysis was performed using anti-TSHR monoclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse ovary lysate