Anti-Human TSHR Recombinant Antibody (K1-70) (HB852207)

Name: Anti-Human TSHR Recombinant Antibody (K1-70)
Brand: abinScience
SKU: HB852207
Price: 191 USD
Availability: InStock

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概要
イメージ
参考文献
データシート
SDS

概要

カタログ番号	HB852207
種反応性	Human
アプリケーション	Blocking, ELISA, FCM, WB
宿主種	Mouse
クローン性	Monoclonal
アイソタイプ	IgG2a, lambda
クローンID	K1-70
テスト済みアプリケーション	WB, FCM
ターゲット	Thyroid-stimulating hormone receptor, TSH-R, TSHR, Thyrotropin receptor, LGR3, RHD18102
エンドトキシンレベル	Please contact with the lab for this information.
純度	>95% as determined by SDS-PAGE.
精製	Protein A/G purified from cell culture supernatant.
アクセッション番号	P16473
形態	Liquid
保存バッファー	0.01M PBS pH 7.4 データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。
安定性と保存	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
背景	Thyrotropin receptor (TSHR/LGR3) is a ~86 kDa protein. Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. TSHR is coupled to G(s) proteins and mediates the activation of adenylate cyclase. This leads to the generation of cyclic adenosine monophosphate (cAMP), which in turn activates protein kinase A (PKA). PKA subsequently phosphorylates downstream targets involved in thyroid hormone biosynthesis and secretion, including thyroid peroxidase (TPO) and the sodium/iodide symporter (NIS). 1. Costagliola, S. et al. (2002) The EMBO journal 21, 504-13. PMID: 11847099 2. Nakabayashi, K. et al. (2002) The Journal of clinical investigation 109, 1445-52. PMID: 12045258 3. Faust, B. et al. (2022) Nature 609, 846-853. PMID: 35940205 4. Duan, J. et al. (2022) Nature 609, 854-859. PMID: 35940204 5. Morgan, SJ. et al. (2016) Thyroid : official journal of the American Thyroid Association 26, 1794-1803. PMID: 27638195
注意事項	For research use only.

イメージ

Flow Cytometry

Flow-cytometry using anti-human TSHR antibody.TSHR Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TSHR antibody monoclonal antibody (Catalog # HB852207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-TSHR monoclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse ovary lysate