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Anti-Human TUBB/Beta Tubulin Recombinant Antibody (SAA2334) (HY374013)

Anti-Human TUBB/Beta Tubulin Recombinant Antibody (SAA2334)
Anti-Human TUBB/Beta Tubulin Recombinant Antibody (SAA2334)
Anti-Human TUBB/Beta Tubulin Recombinant Antibody (SAA2334)
Anti-Human TUBB/Beta Tubulin Recombinant Antibody (SAA2334)
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概要
カタログ番号HY374013
種反応性Human
アプリケーションELISA, IF, WB
宿主種Mouse
クローン性Monoclonal
アイソタイプIgG2a, kappa
クローンIDSAA2334
ターゲット Tubulin beta chain, Tubulin beta-5 chain, TUBB, TUBB5, β tubulin
エンドトキシンレベル Please contact with the lab for this information.
純度 >95% as determined by SDS-PAGE.
精製 Protein A/G purified from cell culture supernatant.
アクセッション番号 P07437
形態 Liquid
保存バッファー 0.01M PBS pH 7.4

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:2000-1:20000
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Tubulin beta chain (TUBB) is a ~49 kDa protein. Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

注意事項 For research use only
Isotype Control Mouse IgG2a (Fc Silence) Recombinant Isotype Control (MOPC 173) (MF533023)
イメージ
  • Anti-Human TUBB/Beta Tubulin Recombinant Antibody (SAA2334)

    SDS-PAGE

    SDS-PAGE for Anti-Human TUBB/Beta Tubulin Antibody (SAA2334)

  • Anti-Human TUBB/Beta Tubulin Recombinant Antibody (SAA2334)

    Western blot

    Western blot analysis was performed using anti-beta Tubulin monoclonal antibody at 1ug/mL on various samples.
    Lane 1: U251 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: A431 cell lysate
    Lane 5: NIH3T3 cell lysate
    Lane 6: Jurkat cell lysate
    Lane 7: MCF-7 cell lysate
    Lane 8: Lncap cell lysate
    Lane 9: Mouse brain lysate
    Lane 10: Rat brain lysate

参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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