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Anti-Human TUFM Polyclonal Antibody
Anti-Human TUFM Polyclonal Antibody
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Обзор
Каталожный номерHW446014
Реактивность видовHuman, Mouse, Rat
ПримененияELISA, IHC, WB
Хозяинский видRabbit
КлоональностьPolyclonal
ИзотипIgG
Иммуноген E. coli - derived recombinant Human TUFM (Asp67-Gly452).
Цель P43, Elongation factor Tu, mitochondrial, TUFM, EF-Tu
Очистка Purified by antigen affinity column.
Номер доступа P49411
Форма Liquid
Буфер хранения 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта
Применение Разбавление
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Устойчивость и хранение Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Фон

Elongation factor Tu, mitochondrial (TUFM) is a ~49 kDa protein. GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. Participates in mitochondrial translation. Also plays a role in the regulation of autophagy and innate immunity. Recruits ATG5-ATG12 and NLRX1 at mitochondria and serves as a checkpoint of the RIGI-MAVS pathway. In turn, inhibits RLR-mediated type I interferon while promoting autophagy.

1. Lei, Y. et al. (2012) Immunity 36, 933-46. PMID: 22749352
2. Ding, B. et al. (2017) Cell host & microbe 21, 538-547.e4. PMID: 28407488
Примечание For research use only.
Изображения
  • Anti-Human TUFM Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-TUFM polyclonal antibody at 1μg/ml on various samples.
    Lane 1: HepG2 cell lysate
    Lane 2: K562 cell lysate
    Lane 3: Mouse brain lysate
    Lane 4: Mouse kidney lysate
    Lane 5: Mouse liver lysate
    Lane 6: Rat brain lysate
    Lane 7: Rat kidney lysate
    Lane 8: Rat liver lysate

Ссылки
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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