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Anti-Human U2AF2 Polyclonal Antibody (HB779014)

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Anti-Human U2AF2 Polyclonal Antibody
Anti-Human U2AF2 Polyclonal Antibody
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Vista general
Número de catálogoHB779014
Descripción
Anti-Human U2AF2 Polyclonal Antibody (HB779014) is a rabbit polyclonal antibody detecting U2AF2 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human U2AF2 (Pro138-Gly338).
Objetivo Splicing factor U2AF 65 kDa subunit, hU2AF65, hU2AF(65), U2 auxiliary factor 65 kDa subunit, U2AF65, U2AF2, U2 snRNP auxiliary factor large subunit
Purificación Purified by antigen affinity column.
Número de acceso P26368
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Splicing factor U2AF 65 kDa subunit (U2AF2) is a ~53 kDa protein. Plays a role in pre-mRNA splicing and 3'-end processing. By recruiting PRPF19 and the PRP19C/Prp19 complex/NTC/Nineteen complex to the RNA polymerase II C-terminal domain (CTD), and thereby pre-mRNA, may couple transcription to splicing. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly.

1. Millevoi, S. et al. (2006) The EMBO journal 25, 4854-64. PMID: 17024186
2. David, CJ. et al. (2011) Genes & development 25, 972-83. PMID: 21536736
Nota For research use only.
Imágenes
  • Anti-Human U2AF2 Polyclonal Antibody

    Western blot

    Western blot analysis was performed using anti-U2AF2 polyclonal antibody at 1ug/mL on various samples.
    Lane 1: Jurkat cell lysate
    Lane 2: 293T cell lysate
    Lane 3: K562 cell lysate

Referencias
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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