Anti-Human UBE2D3 Polyclonal Antibody (HX067014)

Name: Anti-Human UBE2D3 Polyclonal Antibody
Brand: abinScience
SKU: HX067014
Price: 168 USD
Availability: InStock

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50ug
100ug

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Número de catálogo

HX067014

Descripción

Anti-Human UBE2D3 Polyclonal Antibody (HX067014) is a rabbit polyclonal antibody detecting UBE2D3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactividad de especies

Human, Mouse, Rat

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human UBE2D3 (Met1-Met147).

Objetivo

Ubiquitin-conjugating enzyme E2-17 kDa 3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, UBC5C, UBE2D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin carrier protein D3, Ubiquitin-protein ligase D3, UBCH5C, Ubiquitin-conjugating enzyme E2 D3, E2 ubiquitin-conjugating enzyme D3

Purificación

Purified by antigen affinity column.

Número de acceso

P61077

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Ubiquitin-conjugating enzyme E2 D3 (UBE2D3) is a ~16 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin.

1. Rajendra, R. et al. (2004) The Journal of biological chemistry 279, 36440-4. PMID: 15247280

2. Huang, J. et al. (2004) The Journal of biological chemistry 279, 54110-6. PMID: 15496420

3. Kubori, T. et al. (2008) Molecular microbiology 67, 1307-19. PMID: 18284575

4. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

5. Wenzel, DM. et al. (2011) Nature 474, 105-8. PMID: 21532592

6. Peng, H. et al. (2017) Cell research 27, 657-674. PMID: 28322253

7. Wu, K. et al. (2010) Molecular cell 37, 784-96. PMID: 20347421

8. Gonen, H. et al. (1999) The Journal of biological chemistry 274, 14823-30. PMID: 10329681

Nota

For research use only.

Imágenes

Referencias

Datasheet

Anti-Human UBE2D3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo

Anti-Human UBE2D3 Polyclonal Antibody (HX067014)

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Imágenes

Referencias

Datasheet

SDS