Anti-Human UBE2D3 Polyclonal Antibody (HX067014)

Name: Anti-Human UBE2D3 Polyclonal Antibody
Brand: abinScience
SKU: HX067014
Price: 168 USD
Availability: InStock

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100ug

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SDS

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Numéro de catalogue

HX067014

Description

Anti-Human UBE2D3 Polyclonal Antibody (HX067014) is a rabbit polyclonal antibody detecting UBE2D3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human UBE2D3 (Met1-Met147).

Cible

Ubiquitin-conjugating enzyme E2-17 kDa 3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, UBC5C, UBE2D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin carrier protein D3, Ubiquitin-protein ligase D3, UBCH5C, Ubiquitin-conjugating enzyme E2 D3, E2 ubiquitin-conjugating enzyme D3

Purification

Purified by antigen affinity column.

Numéro d'accès

P61077

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Ubiquitin-conjugating enzyme E2 D3 (UBE2D3) is a ~16 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin.

1. Rajendra, R. et al. (2004) The Journal of biological chemistry 279, 36440-4. PMID: 15247280

2. Huang, J. et al. (2004) The Journal of biological chemistry 279, 54110-6. PMID: 15496420

3. Kubori, T. et al. (2008) Molecular microbiology 67, 1307-19. PMID: 18284575

4. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

5. Wenzel, DM. et al. (2011) Nature 474, 105-8. PMID: 21532592

6. Peng, H. et al. (2017) Cell research 27, 657-674. PMID: 28322253

7. Wu, K. et al. (2010) Molecular cell 37, 784-96. PMID: 20347421

8. Gonen, H. et al. (1999) The Journal of biological chemistry 274, 14823-30. PMID: 10329681

Note

For research use only.

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Fiche technique

Anti-Human UBE2D3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

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Kits d'Essai

Anti-Human UBE2D3 Polyclonal Antibody (HX067014)

Aperçu

Images

Reférences

Fiche technique

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