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| カタログ番号 | HX067014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human UBE2D3 Polyclonal Antibody (HX067014) is a rabbit polyclonal antibody detecting UBE2D3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.
Highlights
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| 種反応性 | Human, Mouse, Rat | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human UBE2D3 (Met1-Met147). | ||||||||
| ターゲット | Ubiquitin-conjugating enzyme E2-17 kDa 3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, UBC5C, UBE2D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin carrier protein D3, Ubiquitin-protein ligase D3, UBCH5C, Ubiquitin-conjugating enzyme E2 D3, E2 ubiquitin-conjugating enzyme D3 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | P61077 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Ubiquitin-conjugating enzyme E2 D3 (UBE2D3) is a ~16 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. 1. Rajendra, R. et al. (2004) The Journal of biological chemistry 279, 36440-4. PMID: 15247280 2. Huang, J. et al. (2004) The Journal of biological chemistry 279, 54110-6. PMID: 15496420 3. Kubori, T. et al. (2008) Molecular microbiology 67, 1307-19. PMID: 18284575 4. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386 5. Wenzel, DM. et al. (2011) Nature 474, 105-8. PMID: 21532592 6. Peng, H. et al. (2017) Cell research 27, 657-674. PMID: 28322253 7. Wu, K. et al. (2010) Molecular cell 37, 784-96. PMID: 20347421 8. Gonen, H. et al. (1999) The Journal of biological chemistry 274, 14823-30. PMID: 10329681 | ||||||||
| 注意事項 | For research use only. |
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