Anti-Human UBE2D3 Polyclonal Antibody (HX067014)

Name: Anti-Human UBE2D3 Polyclonal Antibody
Brand: abinScience
SKU: HX067014
Price: 168 USD
Availability: InStock

Даташит Поделиться продуктом Копировать информацию

Цена(USD): --

Спецификация:

50ug
100ug

Количество:

Свяжитесь с нами

Обзор
Изображения
Ссылки
Даташит
SDS

Обзор

Каталожный номер

HX067014

Описание

Anti-Human UBE2D3 Polyclonal Antibody (HX067014) is a rabbit polyclonal antibody detecting UBE2D3 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human UBE2D3 (Met1-Met147).

Цель

Ubiquitin-conjugating enzyme E2-17 kDa 3, (E3-independent) E2 ubiquitin-conjugating enzyme D3, UBC5C, UBE2D3, Ubiquitin-conjugating enzyme E2(17)KB 3, Ubiquitin carrier protein D3, Ubiquitin-protein ligase D3, UBCH5C, Ubiquitin-conjugating enzyme E2 D3, E2 ubiquitin-conjugating enzyme D3

Очистка

Purified by antigen affinity column.

Номер доступа

P61077

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Устойчивость и хранение

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Фон

Ubiquitin-conjugating enzyme E2 D3 (UBE2D3) is a ~16 kDa protein. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'-linked polyubiquitination. Cooperates with the E2 CDC34 and the SCF(FBXW11) E3 ligase complex for the polyubiquitination of NFKBIA leading to its subsequent proteasomal degradation. Acts as an initiator E2, priming the phosphorylated NFKBIA target at positions 'Lys-21' and/or 'Lys-22' with a monoubiquitin. Ubiquitin chain elongation is then performed by CDC34, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin.

1. Rajendra, R. et al. (2004) The Journal of biological chemistry 279, 36440-4. PMID: 15247280

2. Huang, J. et al. (2004) The Journal of biological chemistry 279, 54110-6. PMID: 15496420

3. Kubori, T. et al. (2008) Molecular microbiology 67, 1307-19. PMID: 18284575

4. David, Y. et al. (2010) The Journal of biological chemistry 285, 8595-604. PMID: 20061386

5. Wenzel, DM. et al. (2011) Nature 474, 105-8. PMID: 21532592

6. Peng, H. et al. (2017) Cell research 27, 657-674. PMID: 28322253

7. Wu, K. et al. (2010) Molecular cell 37, 784-96. PMID: 20347421

8. Gonen, H. et al. (1999) The Journal of biological chemistry 274, 14823-30. PMID: 10329681

Примечание

For research use only.

Изображения

Ссылки

Даташит

Anti-Human UBE2D3 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Рекомбинантные Белки

Антитела

Наборы для анализа

Anti-Human UBE2D3 Polyclonal Antibody (HX067014)

Обзор

Изображения

Ссылки

Даташит

SDS