Anti-Human UBR5 Polyclonal Antibody (HC972014)

Name: Anti-Human UBR5 Polyclonal Antibody
Brand: abinScience
SKU: HC972014
Price: 168 USD
Availability: InStock

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100ug

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SDS

Aperçu

Numéro de catalogue

HC972014

Description

Anti-Human UBR5 Polyclonal Antibody (HC972014) is a rabbit polyclonal antibody detecting UBR5. Suitable for Human, Mouse, and Rat.

Highlights

●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human UBR5 (Pro640-Ala870).

Cible

E3 ubiquitin-protein ligase UBR5, E3 ubiquitin-protein ligase, HECT domain-containing 1, EC:2.3.2.26, EDD, EDD1, HYD, Hyperplastic discs protein homolog, KIAA0896, Progestin-induced protein, UBR5, hHYD

Niveau d'endotoxines

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Numéro d'accès

O95071

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Contexte

E3 ubiquitin-protein ligase UBR5 is a ~309 kDa protein. E3 ubiquitin-protein ligase involved in different protein quality control pathways in the cytoplasm and nucleus. Mainly acts as a ubiquitin chain elongator that extends pre-ubiquitinated substrates. Component of the N-end rule pathway: ubiquitinates proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their degradation. Recognizes type-1 N-degrons, containing positively charged amino acids (Arg, Lys and His). Together with UBR4, part of a cytoplasm protein quality control pathway that prevents protein aggregation by catalyzing assembly of heterotypic 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on aggregated proteins, leading to substrate recognition by the segregase p97/VCP and degradation by the proteasome: UBR5 is probably branching multiple 'Lys-48'-linked chains of substrates initially modified with mixed conjugates by UBR4.

1. Yau, RG. et al. (2017) Cell 171, 918-933.e20. PMID: 29033132

2. Schukur, L. et al. (2020) Scientific reports 10, 20044. PMID: 33208877

3. Tsai, JM. et al. (2023) Molecular cell 83, 2753-2767.e10. PMID: 37478846

4. Mark, KG. et al. (2023) Cell 186, 3460-3475.e23. PMID: 37478862

5. Hodáková, Z. et al. (2023) The EMBO journal 42, e113348. PMID: 37409633

6. Ohtake, F. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1401-E1408. PMID: 29378950

10. Jiang, W. et al. (2011) Molecular cell 43, 33-44. PMID: 21726808

Note

For research use only

Images

Reférences

Fiche technique

Anti-Human UBR5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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