Anti-Human UBR5 Polyclonal Antibody (HC972014)

Name: Anti-Human UBR5 Polyclonal Antibody
Brand: abinScience
SKU: HC972014
Price: 168 USD
Availability: InStock

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Обзор

Каталожный номер

HC972014

Описание

Anti-Human UBR5 Polyclonal Antibody (HC972014) is a rabbit polyclonal antibody detecting UBR5. Suitable for Human, Mouse, and Rat.

Highlights

●Multi-Species — Cross-reactive for translational research.

Реактивность видов

Human, Mouse, Rat

Применения

ELISA, IHC, WB

Хозяинский вид

Rabbit

Клоональность

Polyclonal

Изотип

IgG

Иммуноген

E. coli - derived recombinant Human UBR5 (Pro640-Ala870).

Цель

E3 ubiquitin-protein ligase UBR5, E3 ubiquitin-protein ligase, HECT domain-containing 1, EC:2.3.2.26, EDD, EDD1, HYD, Hyperplastic discs protein homolog, KIAA0896, Progestin-induced protein, UBR5, hHYD

Уровень эндотоксинов

Please contact with the lab for this information.

Очистка

Purified by antigen affinity column.

Номер доступа

O95071

Форма

Liquid

Буфер хранения

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.

Информация об использовании продукта

Применение	Разбавление
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Фон

E3 ubiquitin-protein ligase UBR5 is a ~309 kDa protein. E3 ubiquitin-protein ligase involved in different protein quality control pathways in the cytoplasm and nucleus. Mainly acts as a ubiquitin chain elongator that extends pre-ubiquitinated substrates. Component of the N-end rule pathway: ubiquitinates proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their degradation. Recognizes type-1 N-degrons, containing positively charged amino acids (Arg, Lys and His). Together with UBR4, part of a cytoplasm protein quality control pathway that prevents protein aggregation by catalyzing assembly of heterotypic 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on aggregated proteins, leading to substrate recognition by the segregase p97/VCP and degradation by the proteasome: UBR5 is probably branching multiple 'Lys-48'-linked chains of substrates initially modified with mixed conjugates by UBR4.

1. Yau, RG. et al. (2017) Cell 171, 918-933.e20. PMID: 29033132

2. Schukur, L. et al. (2020) Scientific reports 10, 20044. PMID: 33208877

3. Tsai, JM. et al. (2023) Molecular cell 83, 2753-2767.e10. PMID: 37478846

4. Mark, KG. et al. (2023) Cell 186, 3460-3475.e23. PMID: 37478862

5. Hodáková, Z. et al. (2023) The EMBO journal 42, e113348. PMID: 37409633

6. Ohtake, F. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, E1401-E1408. PMID: 29378950

10. Jiang, W. et al. (2011) Molecular cell 43, 33-44. PMID: 21726808

Примечание

For research use only

Изображения

Ссылки

Даташит

Anti-Human UBR5 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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