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| カタログ番号 | HP185024 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human UHRF1 Polyclonal Antibody (HP185024) is a rabbit polyclonal antibody detecting UHRF1 in ELISA, IHC, WB. Suitable for Bovine, Danio rerio, Human, Xenopus laevis, and Xenopus tropicalis.
Highlights
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| 種反応性 | Human | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human UHRF1 (Gly427-Gly636). | ||||||||
| ターゲット | Nuclear zinc finger protein Np95, RING-type E3 ubiquitin transferase UHRF1, RING finger protein 106, Transcription factor ICBP90, E3 ubiquitin-protein ligase UHRF1, Ubiquitin-like PHD and RING finger domain-containing protein 1, NP95, HuNp95, Nuclear protein 95, RNF106, ICBP90, hNp95, UHRF1, Inverted CCAAT box-binding protein of 90 kDa, hUHRF1, Ubiquitin-like-containing PHD and RING finger domains protein 1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | Q96T88 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | E3 ubiquitin-protein ligase UHRF1 is a ~89 kDa protein. E3 ubiquitin-protein ligase that acts as a key epigenetic regulator by bridging DNA methylation and chromatin modification. Plays a key role in DNA methylation inheritance by promoting recruitment of DNMT1 to hemimethylated DNA and ensure faithful propagation of the DNA methylation patterns through DNA replication. Acts both as a histone reader and writer: specifically recognizes and binds (1) hemimethylated DNA at replication forks and (2) histone H3 trimethylated at 'Lys-9' and unmethylated at 'Arg-2' (H3K9me3 and H3R2me0, respectively), thereby activating its E3 ubiquitin-protein ligase activity. UHRF1 then mediates histone H3 'Lys-18' monoubiquitination (H3K18ub), a docking site for DNMT1, leading to DNMT1 recruitment and replication-coupled DNA methylation maintenance. Also mediates histone H3 'Lys-14' and 'Lys-23' ubiquitination (H3K14ub and H3K23ub) at lower level. 1. Hopfner, R. et al. (2000) Cancer research 60, 121-8. PMID: 10646863 2. Arima, Y. et al. (2004) Genes to cells : devoted to molecular & cellular mechanisms 9, 131-42. PMID: 15009091 3. Kim, JK. et al. (2009) Nucleic acids research 37, 493-505. PMID: 19056828 4. Rothbart, SB. et al. (2012) Nature structural & molecular biology 19, 1155-60. PMID: 23022729 5. Nishiyama, A. et al. (2013) Nature 502, 249-53. PMID: 24013172 6. Harrison, JS. et al. (2016) eLife 5. PMID: 27595565 7. Vaughan, RM. et al. (2018) Proceedings of the National Academy of Sciences of the United States of America 115, 8775-8780. PMID: 30104358 8. Foster, BM. et al. (2018) Molecular cell 72, 739-752.e9. PMID: 30392929 9. DaRosa, PA. et al. (2018) Molecular cell 72, 753-765.e6. PMID: 30392931 10. Tiedemann, RL. et al. (2024) Nucleic acids research 52, 13733-13756. PMID: 39607687 | ||||||||
| 注意事項 | For research use only. |
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