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| カタログ番号 | HF942014 | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 説明 |
Anti-Human ULK1 Polyclonal Antibody (HF942014) is a rabbit polyclonal antibody detecting ULK1 in ELISA, IHC, WB. Suitable for Human.
Highlights
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| 種反応性 | Human, Mouse | ||||||||
| アプリケーション | ELISA, IHC, WB | ||||||||
| 宿主種 | Rabbit | ||||||||
| クローン性 | Polyclonal | ||||||||
| アイソタイプ | IgG | ||||||||
| 免疫原 | E. coli - derived recombinant Human ULK1 (Met1-His275). | ||||||||
| ターゲット | ULK1, Autophagy-related protein 1 homolog, Unc-51-like kinase 1, KIAA0722, Serine/threonine-protein kinase ULK1, hATG1, ATG1 | ||||||||
| 精製 | Purified by antigen affinity column. | ||||||||
| アクセッション番号 | O75385 | ||||||||
| 形態 | Liquid | ||||||||
| 保存バッファー | 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
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| 製品使用情報 |
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| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. | ||||||||
| 背景 | Serine/threonine-protein kinase ULK1 is a ~112 kDa protein. Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. 1. Chan, EY. et al. (2009) Molecular and cellular biology 29, 157-71. PMID: 18936157 2. Löffler, AS. et al. (2011) Autophagy 7, 696-706. PMID: 21460634 3. Jung, CH. et al. (2011) Autophagy 7, 1212-21. PMID: 21795849 4. Nazio, F. et al. (2013) Nature cell biology 15, 406-16. PMID: 23524951 5. Ro, SH. et al. (2014) The FEBS journal 281, 3816-27. PMID: 25040165 6. Kim, JH. et al. (2018) EMBO reports 19. PMID: 29487085 7. Di Rienzo, M. et al. (2019) Science advances 5, eaau8857. PMID: 31123703 | ||||||||
| 注意事項 | For research use only. |
Western blot analysis was performed using anti-ULK1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse brain lysate
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