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Anti-Human UNG Polyclonal Antibody (HY404014)

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Vista general
Número de catálogoHY404014
Descripción
Anti-Human UNG Polyclonal Antibody (HY404014) is a rabbit polyclonal antibody detecting UNG. Suitable for Human.
Reactividad de especiesHuman
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human UNG (Ala45-Leu313).
Objetivo DGU, EC:3.2.2.27, UDG, UNG, UNG1, UNG15, Uracil-DNA glycosylase
Nivel de endotoxinas Please contact with the lab for this information.
Purificación Purified by antigen affinity column.
Número de acceso P13051
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Fondo

Uracil-DNA glycosylase (UNG) is a ~34 kDa protein. Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine. Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication.

1. Imai, K. et al. (2003) Nature immunology 4, 1023-8. PMID: 12958596
2. Kavli, B. et al. (2005) The Journal of experimental medicine 201, 2011-21. PMID: 15967827
3. Akbari, M. et al. (2007) Neuroscience 145, 1201-12. PMID: 17101234
4. Torseth, K. et al. (2012) DNA repair 11, 559-69. PMID: 22521144
5. Mol, CD. et al. (1995) Cell 82, 701-8. PMID: 7671300
6. Slupphaug, G. et al. (1996) Nature 384, 87-92. PMID: 8900285
7. Nilsen, H. et al. (1997) Nucleic acids research 25, 750-5. PMID: 9016624
8. Bharati, S. et al. (1998) Nucleic acids research 26, 4953-9. PMID: 9776759
Nota For research use only
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human UNG Polyclonal Antibody

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