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Anti-Human UNG Polyclonal Antibody (HY404014)

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Aperçu
Numéro de catalogueHY404014
Description
Anti-Human UNG Polyclonal Antibody (HY404014) is a rabbit polyclonal antibody detecting UNG. Suitable for Human.
Reactivité des espècesHuman
ApplicationsELISA, IHC, WB
Espèce hôteRabbit
ClonalitéPolyclonal
IsotypeIgG
Immunogène E. coli - derived recombinant Human UNG (Ala45-Leu313).
Cible DGU, EC:3.2.2.27, UDG, UNG, UNG1, UNG15, Uracil-DNA glycosylase
Niveau d'endotoxines Please contact with the lab for this information.
Purification Purified by antigen affinity column.
Numéro d'accès P13051
Forme Liquid
Tampon de stockage 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Contexte

Uracil-DNA glycosylase (UNG) is a ~34 kDa protein. Uracil-DNA glycosylase that hydrolyzes the N-glycosidic bond between uracil and deoxyribose in single- and double-stranded DNA (ssDNA and dsDNA) to release a free uracil residue and form an abasic (apurinic/apyrimidinic; AP) site. Excises uracil residues arising as a result of misincorporation of dUMP residues by DNA polymerase during replication or due to spontaneous or enzymatic deamination of cytosine. Mediates error-free base excision repair (BER) of uracil at replication forks. According to the model, it is recruited by PCNA to S-phase replication forks to remove misincorporated uracil at U:A base mispairs in nascent DNA strands. Via trimeric RPA it is recruited to ssDNA stretches ahead of the polymerase to allow detection and excision of deaminated cytosines prior to replication.

1. Imai, K. et al. (2003) Nature immunology 4, 1023-8. PMID: 12958596
2. Kavli, B. et al. (2005) The Journal of experimental medicine 201, 2011-21. PMID: 15967827
3. Akbari, M. et al. (2007) Neuroscience 145, 1201-12. PMID: 17101234
4. Torseth, K. et al. (2012) DNA repair 11, 559-69. PMID: 22521144
5. Mol, CD. et al. (1995) Cell 82, 701-8. PMID: 7671300
6. Slupphaug, G. et al. (1996) Nature 384, 87-92. PMID: 8900285
7. Nilsen, H. et al. (1997) Nucleic acids research 25, 750-5. PMID: 9016624
8. Bharati, S. et al. (1998) Nucleic acids research 26, 4953-9. PMID: 9776759
Note For research use only
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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