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Anti-Human USP1 Polyclonal Antibody (HF660014)

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概要
カタログ番号HF660014
説明
Anti-Human USP1 Polyclonal Antibody (HF660014) is a rabbit polyclonal antibody detecting USP1 in ELISA, IHC, WB. Suitable for Human, Mouse, Bovine, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
種反応性Human, Mouse, Rat
アプリケーションELISA, IHC, WB
宿主種Rabbit
クローン性Polyclonal
アイソタイプIgG
免疫原 E. coli - derived recombinant Human USP1 (Arg486-Lys599).
ターゲット Ubiquitin thioesterase 1, Ubiquitin carboxyl-terminal hydrolase 1, Deubiquitinating enzyme 1, Ubiquitin-specific-processing protease 1, USP1, hUBP
精製 Purified by antigen affinity column.
アクセッション番号 O94782
形態 Liquid
保存バッファー 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

製品使用情報
アプリケーション 希釈
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
背景

Ubiquitin carboxyl-terminal hydrolase 1 (USP1) is a ~88 kDa protein. Negative regulator of DNA damage repair which specifically deubiquitinates monoubiquitinated FANCD2. Also involved in PCNA-mediated translesion synthesis (TLS) by deubiquitinating monoubiquitinated PCNA. Has almost no deubiquitinating activity by itself and requires the interaction with WDR48 to have a high activity.

1. Nijman, SM. et al. (2005) Molecular cell 17, 331-9. PMID: 15694335
2. Huang, TT. et al. (2006) Nature cell biology 8, 339-47. PMID: 16531995
3. Lee, KY. et al. (2010) The Journal of biological chemistry 285, 10362-9. PMID: 20147293
4. Cohn, MA. et al. (2007) Molecular cell 28, 786-97. PMID: 18082604
5. Yin, J. et al. (2015) Structure (London, England : 1993) 23, 2043-54. PMID: 26388029
注意事項 For research use only.
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参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
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Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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Working Solution
C₂ (Working Conc.)
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