Anti-Human USP10 Polyclonal Antibody (HC297014)

Name: Anti-Human USP10 Polyclonal Antibody
Brand: abinScience
SKU: HC297014
Price: 168 USD
Availability: InStock

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100ug

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Numéro de catalogue

HC297014

Description

Anti-Human USP10 Polyclonal Antibody (HC297014) is a rabbit polyclonal antibody detecting USP10 in ELISA, IHC, WB. Suitable for Human, Mouse, Rat.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human USP10 (Thr600-Leu798).

Cible

Ubiquitin thioesterase 10, USP10, Ubiquitin carboxyl-terminal hydrolase 10, Ubiquitin-specific-processing protease 10, Deubiquitinating enzyme 10, KIAA0190

Purification

Purified by antigen affinity column.

Numéro d'accès

Q14694

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Ubiquitin carboxyl-terminal hydrolase 10 (USP10) is a ~87 kDa protein. Hydrolase that can remove conjugated ubiquitin from target proteins such as p53/TP53, RPS2/us5, RPS3/us3, RPS10/eS10, BECN1, SNX3 and CFTR. Acts as an essential regulator of p53/TP53 stability: in unstressed cells, specifically deubiquitinates p53/TP53 in the cytoplasm, leading to counteract MDM2 action and stabilize p53/TP53. Following DNA damage, translocates to the nucleus and deubiquitinates p53/TP53, leading to regulate the p53/TP53-dependent DNA damage response. Component of a regulatory loop that controls autophagy and p53/TP53 levels: mediates deubiquitination of BECN1, a key regulator of autophagy, leading to stabilize the PIK3C3/VPS34-containing complexes. In turn, PIK3C3/VPS34-containing complexes regulate USP10 stability, suggesting the existence of a regulatory system by which PIK3C3/VPS34-containing complexes regulate p53/TP53 protein levels via USP10 and USP13.

1. Soncini, C. et al. (2001) Oncogene 20, 3869-79. PMID: 11439350

2. Boulkroun, S. et al. (2008) American journal of physiology. Renal physiology 295, F889-900. PMID: 18632802

3. Meyer, C. et al. (2020) Molecular cell 77, 1193-1205.e5. PMID: 31981475

4. Yuan, J. et al. (2010) Cell 140, 384-96. PMID: 20096447

5. Liu, J. et al. (2011) Cell 147, 223-34. PMID: 21962518

6. Kedersha, N. et al. (2016) The Journal of cell biology 212, 845-60. PMID: 27022092

9. Sanders, DW. et al. (2020) Cell 181, 306-324.e28. PMID: 32302570

Note

For research use only.

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Fiche technique

Anti-Human USP10 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Human USP10 Polyclonal Antibody (HC297014)

Aperçu

Images

Reférences

Fiche technique

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