Anti-Human USP22 Polyclonal Antibody (HP083014)

Name: Anti-Human USP22 Polyclonal Antibody
Brand: abinScience
SKU: HP083014
Price: 96 USD
Availability: InStock

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Contáctenos

Vista general
Imágenes
Referencias
Datasheet
SDS

Vista general

Número de catálogo

HP083014

Reactividad de especies

Human, Mouse

Aplicaciones

ELISA, IHC, WB

Especie huésped

Rabbit

Clonalidad

Polyclonal

Isotipo

IgG

Inmunógeno

E. coli - derived recombinant Human USP22 (Gly182-Glu525).

Objetivo

Deubiquitinating enzyme 22, USP22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, USP3L, Ubiquitin-specific-processing protease 22

Purificación

Purified by antigen affinity column.

Número de acceso

Q9UPT9

Forma

Liquid

Buffer de almacenamiento

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Información de uso del producto

Aplicación	Dilución
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Estabilidad y almacenamiento

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Fondo

Ubiquitin carboxyl-terminal hydrolase 22 (USP22) is a ~59 kDa protein. Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. As part of the transcription regulatory histone acetylation (HAT) complex SAGA, catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a transcriptional coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Facilitates cell-cycle progression by stabilizing CCNB1 and antagonizing its proteasome-mediated degradation in a cell cycle-specific manner. Modulates cell cycle progression and apoptosis also by antagonizing TP53 transcriptional activation through deacetylase SIRT1 stabilization.

1. Zhao, Y. et al. (2008) Molecular cell 29, 92-101. PMID: 18206972

2. Zhang, XY. et al. (2008) Molecular cell 29, 102-11. PMID: 18206973

3. Zhang, XY. et al. (2008) Cell cycle (Georgetown, Tex.) 7, 1522-4. PMID: 18469533

4. Lin, Z. et al. (2015) Cell discovery 1, 15028-. PMID: 27030811

5. Lin, Z. et al. (2012) Molecular cell 46, 484-94. PMID: 22542455

6. Cai, Z. et al. (2020) The Journal of experimental medicine 217. PMID: 32130408

7. Karlowitz, R. et al. (2022) Cell death & disease 13, 684. PMID: 35933402

8. Huang, X. et al. (2019) Cancer immunology research 7, 1580-1590. PMID: 31399419

9. Roedig, J. et al. (2021) EMBO reports 22, e50163. PMID: 33369872

Nota

For research use only.

Imágenes

Western blot

Western blot analysis was performed using anti-USP22 polyclonal antibody at 1µg/ml on various samples.
Lane 1: HepG2 cell lysate

Referencias

Datasheet

Anti-Human USP22 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Proteínas Recombinantes

Anticuerpos

Kits de Ensayo