Anti-Human USP22 Polyclonal Antibody (HP083014)

Name: Anti-Human USP22 Polyclonal Antibody
Brand: abinScience
SKU: HP083014
Price: 96 USD
Availability: InStock

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Aperçu
Images
Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HP083014

Reactivité des espèces

Human, Mouse

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human USP22 (Gly182-Glu525).

Cible

Deubiquitinating enzyme 22, USP22, Ubiquitin thioesterase 22, Ubiquitin carboxyl-terminal hydrolase 22, KIAA1063, USP3L, Ubiquitin-specific-processing protease 22

Purification

Purified by antigen affinity column.

Numéro d'accès

Q9UPT9

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Ubiquitin carboxyl-terminal hydrolase 22 (USP22) is a ~59 kDa protein. Deubiquitinase that plays a role in several cellular processes including transcriptional regulation, cell cycle progression or innate immunity. As part of the transcription regulatory histone acetylation (HAT) complex SAGA, catalyzes the deubiquitination of both histones H2A and H2B, thereby acting as a transcriptional coactivator. Recruited to specific gene promoters by activators such as MYC, where it is required for transcription. Facilitates cell-cycle progression by stabilizing CCNB1 and antagonizing its proteasome-mediated degradation in a cell cycle-specific manner. Modulates cell cycle progression and apoptosis also by antagonizing TP53 transcriptional activation through deacetylase SIRT1 stabilization.

1. Zhao, Y. et al. (2008) Molecular cell 29, 92-101. PMID: 18206972

2. Zhang, XY. et al. (2008) Molecular cell 29, 102-11. PMID: 18206973

3. Zhang, XY. et al. (2008) Cell cycle (Georgetown, Tex.) 7, 1522-4. PMID: 18469533

4. Lin, Z. et al. (2015) Cell discovery 1, 15028-. PMID: 27030811

5. Lin, Z. et al. (2012) Molecular cell 46, 484-94. PMID: 22542455

6. Cai, Z. et al. (2020) The Journal of experimental medicine 217. PMID: 32130408

7. Karlowitz, R. et al. (2022) Cell death & disease 13, 684. PMID: 35933402

8. Huang, X. et al. (2019) Cancer immunology research 7, 1580-1590. PMID: 31399419

9. Roedig, J. et al. (2021) EMBO reports 22, e50163. PMID: 33369872

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-USP22 polyclonal antibody at 1µg/ml on various samples.
Lane 1: HepG2 cell lysate

Reférences

Fiche technique

Anti-Human USP22 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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