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Anti-Human USP30 Polyclonal Antibody (HA920014)

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Número de catálogoHA920014
Descripción
Anti-Human USP30 Polyclonal Antibody (HA920014) is a rabbit polyclonal antibody detecting USP30 in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Reactividad de especiesHuman, Mouse, Rat
AplicacionesELISA, IHC, WB
Especie huéspedRabbit
ClonalidadPolyclonal
IsotipoIgG
Inmunógeno E. coli - derived recombinant Human USP30 (Gly74-Lys355).
Objetivo Ubiquitin carboxyl-terminal hydrolase 30, 3.4.19.12, Deubiquitinating enzyme 30, Ubiquitin thioesterase 30, Ubiquitin-specific-processing protease 30, Ub-specific protease 30, USP30
Purificación Purified by antigen affinity column.
Número de acceso Q70CQ3
Forma Liquid
Buffer de almacenamiento 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Información de uso del producto
Aplicación Dilución
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Fondo

Ubiquitin carboxyl-terminal hydrolase 30 (USP30) is a ~58 kDa protein. Deubiquitinating enzyme tethered to the mitochondrial outer membrane that acts as a key inhibitor of mitophagy by counteracting the action of parkin (PRKN): hydrolyzes ubiquitin attached by parkin on target proteins, such as RHOT1/MIRO1 and TOMM20, thereby blocking parkin's ability to drive mitophagy. Preferentially cleaves 'Lys-6'- and 'Lys-11'-linked polyubiquitin chains, 2 types of linkage that participate in mitophagic signaling. Does not cleave efficiently polyubiquitin phosphorylated at 'Ser-65'. Acts as a negative regulator of mitochondrial fusion by mediating deubiquitination of MFN1 and MFN2.

1. Nakamura, N. et al. (2008) Molecular biology of the cell 19, 1903-11. PMID: 18287522
2. Bingol, B. et al. (2014) Nature 510, 370-5. PMID: 24896179
3. Wauer, T. et al. (2015) The EMBO journal 34, 307-25. PMID: 25527291
4. Cunningham, CN. et al. (2015) Nature cell biology 17, 160-9. PMID: 25621951
Nota For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
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Concentration
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Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
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V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
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V₂ (Working Vol.)
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Anti-Human USP30 Polyclonal Antibody

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