Anti-Human USP4 Polyclonal Antibody (HC280014)

Name: Anti-Human USP4 Polyclonal Antibody
Brand: abinScience
SKU: HC280014
Price: 96 USD
Availability: InStock

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50ug
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Reférences
Fiche technique
SDS

Aperçu

Numéro de catalogue

HC280014

Description

Anti-Human USP4 Polyclonal Antibody (HC280014) is a rabbit polyclonal antibody detecting USP4 in ELISA, IHC, WB. Suitable for Human, Bovine, Rat, and Mouse.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Reactivité des espèces

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Espèce hôte

Rabbit

Clonalité

Polyclonal

Isotype

IgG

Immunogène

E. coli - derived recombinant Human USP4 (Asp12-Glu220).

Cible

Ubiquitin carboxyl-terminal hydrolase 4, 3.4.19.12, Deubiquitinating enzyme 4, Ubiquitin thioesterase 4, Ubiquitin-specific-processing protease 4, Ubiquitous nuclear protein homolog, USP4, UNP, UNPH

Niveau d'endotoxines

Please contact with the lab for this information.

Masse moléculaire observée

~110 kDa

Purification

Purified by antigen affinity column.

Numéro d'accès

Q13107, A6QR55, B2GUZ1, P35123

Forme

Liquid

Tampon de stockage

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.

Informations d'utilisation du produit

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stabilité et stockage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Contexte

Ubiquitin carboxyl-terminal hydrolase 4 (USP4) is a ~108 kDa protein. Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. Deubiquitinates HAS2.

1. Wada, K. et al. (2006) Biochemical and biophysical research communications 339, 731-6. PMID: 16316627

2. Milojevic, T. et al. (2006) Molecular pharmacology 69, 1083-94. PMID: 16339847

3. Wada, K. et al. (2006) Biochemical and biophysical research communications 342, 253-8. PMID: 16472766

4. Song, EJ. et al. (2010) Genes & development 24, 1434-47. PMID: 20595234

5. Uras, IZ. et al. (2012) PloS one 7, e31003. PMID: 22347420

6. Clerici, M. et al. (2014) Nature communications 5, 5399. PMID: 25404403

7. Mehić, M. et al. (2017) Oncogenesis 6, e348. PMID: 28604766

8. Deng, L. et al. (2019) Cell research 29, 136-150. PMID: 30514904

9. Chu, F. et al. (2024) Proceedings of the National Academy of Sciences of the United States of America 121, e2401174121. PMID: 39589880

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-USP4 polyclonal antibody at 1ug/mL on various samples.
Lane 1: Hela cell lysate
Lane 2: Jurkat cell lysate

Reférences

Fiche technique

Anti-Human USP4 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Protéines Recombinantes

Anticorps

Kits d'Essai

Anti-Human USP4 Polyclonal Antibody (HC280014)

Aperçu

Images

Reférences

Fiche technique

SDS